Reporter
Part:BBa_K1997005:Design
Designed by: Xinyuan Qiu Group: iGEM16_NUDT_CHINA (2016-10-13)
sHRP-N->FRB->RBS->FKBP-> sHRP-C
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 154
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 396
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1324
Illegal BsaI.rc site found at 679
Illegal SapI.rc site found at 1438
Design Notes
This part is codon optimized for expression in E.coli This part is codon optimized for expression in E.coli, a 10aa linker was added to the both sides of the cds
Source
This part is custom synthesized, the original sequence was acquired form firefly