Reporter

Part:BBa_K1997005:Design

Designed by: Xinyuan Qiu   Group: iGEM16_NUDT_CHINA   (2016-10-13)


sHRP-N->FRB->RBS->FKBP-> sHRP-C


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 154
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1324
    Illegal BsaI.rc site found at 679
    Illegal SapI.rc site found at 1438


Design Notes

This part is codon optimized for expression in E.coli This part is codon optimized for expression in E.coli, a 10aa linker was added to the both sides of the cds



Source

This part is custom synthesized, the original sequence was acquired form firefly

References